Abstract: In order to obtain the main influencing factors of buffeting response calculation of Π‑shaped main girder cable‑stayed bridge， taking Qingzhou Minjiang River Bridge as the engineering background， the buffeting response characteristics of main girder displacement were analyzed by using three‑dimensional multi‑modal coupling buffeting calculation method on the basis of wind tunnel test. The significance of nine factors in buffeting response was tested by uniform experimental design and regression analysis method. The results show that the aerodynamic admittance， fluctuating wind correlation coefficient， vertical wind spectrum， average wind profile index， surface roughness height and air density have significant influence on the buffeting response of cable‑stayed bridge in the common range of values. The influence of structural mass and damping ratio is not significant， and its value deviation can be ignored in buffeting calculation. The horizontal wind spectrum only has a significant effect on the lateral buffeting response.