Abstract: The human body’s perception of one stimulus may change due to the occurrence of another stimulus， which leads to the possibility of a masking effect between vibrations that successively act on the human body. Whether the masking effect is significant or not is related to many factors. This study designed and carried out a seated human vibration experiment to explore the impact of time-domain masking effects on vibration comfort of the seated human body. Designed variables include： intensity of masking signals （3 levels）， vibration interval （4 levels）， and masking sequence （forward and backward）. The experimental results show that these factors do have an impact on the overall comfort when subjected to whole-body vibration； comparing the effects of forward masking tests and backward masking tests， the discomfort caused by backward masking tests is stronger； with the increase of vibration time interval and the decrease of the masking signal strength， the influence of the masking effect on human body discomfort gradually weakens. It can be found that in addition to frequency domain weighting， the time domain characteristics of vibration amplitude also have a non-negligible impact on the evaluation of human vibration discomfort. Comfort prediction based on objective responses should consider the influence of more factors.