Abstract: The deflection influence line and strain influence line can integrally reflect the flexural stiffness of beam bridge section. In the process of obtaining the measured time-history response of beam bridge， the response of beam bridge involves the influence line information and structural dynamic components， and is interfered by the multi-axis effect of loading vehicle under vehicle moving load. In order to identify the influence line of beam bridge structure accurately， the empirical mode decomposition was proposed to eliminate the dynamic component in the measured data of beam bridge， and the quasi-static response data of beam bridge containing the multi-axis effect was obtained. Combined with the sampling frequency and vehicle wheelbase， a mathematical model was estab lished to identify the influence line， and the multi-axis effect of vehicle was converted into unit concentrated load. Tikhonov regular ization method was used to accurately solve the stable solution of the influence line of the beam bridge. Through the establishment of numerical simulation models of 1/2 two-axle vehicle-crossing simply-supported beam bridge and three-span continuous beam